Past Project
Dielectrophoresis Field-Flow Fractionation System
for Detection of Aquatic Toxicants
dFFF chamberSittisak Pui-ock,†
Laboratory
of Environmental Toxicology, Chulabhorn Research
Institute, Bangkok, Thailand, Department of Pharmacology, Faculty of Science, Mahidol University, Bangkok, Thailand, and Department of
Molecular Pathology, M.D. Anderson Cancer Center, University of Texas, Houston,
Texas 77030
Received
for review May 29, 2008. Accepted July 11, 2008.
Dielectrophoretic
field-flow fractionation (dFFF) was applied as a
contact-free way to sense changes in the plasma membrane capacitances and
conductivities of cultured human HL-60 cells in response to toxicant exposure.
A micropatterned electrode imposed electric forces on
cells in suspension in a parabolic flow profile as they moved through a thin
chamber. Relative changes in the dFFF peak elution
time, reflecting changes in cell membrane area and ion permeability, were
measured as indices of response during the first 150 min of exposure to eight
toxicants having different single or mixed modes of action (acrylonitrile,
actinomycin D, carbon tetrachloride, endosulfan, N-nitroso-N-methylurea
(NMU), paraquat dichloride, puromycin,
and styrene oxide). The dFFF method was compared with
the cell viability assay for all toxicants and with the mitochondrial potentiometric dye assay or DNA alkaline comet assay
according to the mode of action of the specific agents. Except for low doses of
nucleic acid-targeting agents (actinomycin D and
NMU), the dFFF method detected all toxicants more
sensitively than other assays, in some cases up to 105 times more
sensitively than the viability approach. The results suggest the dFFF method merits additional study for possible
applicability in toxicology.
For full detail of the
current paper, please visit http://pubs.acs.org/cgi-bin/abstract.cgi/ancham/2008/80/i20/abs/ac801095p.html
อ่านบทความการพัฒนาวิธีการ dFFF กดเพื่อดาวโหลด Click here to download a dFFF article
อ่านวิทยานิพนธ์เรื่อง "Application of Dielectrophoretic Field-Flow Fractionation in Environmental Studies" กดเพื่อดาวโหลด Click here to download Ph.D. Dissertation
สำหรับท่านที่ต้องการอ่านเป็นภาษาไทย Read more on Dielectrophoresis
(in Thai) please ให้กดที่นี่ Click here
